p28 peptide  (MultiTarget Pharmaceuticals)

Journal: Cell Death & Disease

Article Title: Fatostatin induces ferroptosis through inhibition of the AKT/mTORC1/GPX4 signaling pathway in glioblastoma

doi: 10.1038/s41419-023-05738-8

Figure Lengend Snippet: A The chemical formula of fatostatin, PLGA, and PLGA-PEG-MAL. B Schematic diagram of nanoparticle synthesis. C SEM showed the morphology of NPs-FAT (loaded with fatostatin) and p28-NPs-FAT. D The ζ-potential of NPs-FAT and p28-NPs-FAT. E The mean hydration diameter of NPs-FAT and p28-NPs-FAT. F Cellular uptake assay using free C6, NPs encapsulated C6, and p28-NPs encapsulated C6. G CCK-8 assays showed the cell viability (absorbance value at 450 nm) of U87 and U251 cells after treatment with PBS, fatostatin (FAT), NPs-FAT, or p28-NPs-FAT. H The invasion ability of U87 and U251 cells after treatment with PBS (I), FAT(II), NPs-FAT(III), or p28-NPs-FAT (IV). I The ROS levels of U87 and U251 cells after treatment with PBS, FAT, NPs-FAT, or p28-NPs-FAT.

Article Snippet: P28 peptide (sequence: LSTAADMQGVVTDGMASGLDKDYLKPDDC) was purchased from GenScript.

Techniques: CCK-8 Assay

Journal: Cell Death & Disease

Article Title: Fatostatin induces ferroptosis through inhibition of the AKT/mTORC1/GPX4 signaling pathway in glioblastoma

doi: 10.1038/s41419-023-05738-8

Figure Lengend Snippet: A Representative IVIS imaging of the tumor-bearing mice treated with free IR780 (control), NPs loaded with IR780, and p28-NPs loaded with IR780, respectively. The left image is luciferase fluorescence indicating the tumor size, and the right image is the IR780 signal indicating the accumulation of IR780 particles. B IVIS imaging of the isolated organs showed the IR780 signal in the brain, heart, liver, spleen, lung, and kidney of mice receiving the corresponding treatments. C The semiquantification of the NPs-IR780 signal from the isolated organs. D The controlled release of p28-NPs-FAT in PBS. E The plasma concentration of RhoB was detected at predetermined time intervals after intravenous injection of p28-NPs loaded with RhoB. F Kaplan‒Meier curves showing the survival of the untreated or treated mice in the experimental groups. ( 1 p: comparing the PBS with fatostatin (FAT) treatment group; 2 p: comparing the PBS with NPs-FAT treatment group; 3 p: comparing the PBS with p28-NPs-FAT treatment group.) G Representative bioluminescence images from IVIS imaging showed the tumor luciferase signal in the mice of different treatment groups at 1, 3, and 5 weeks. H Representative images of H&E staining of brain sections of the different groups. I Representative IHC images showing the expression levels of p-AKT, p-mTOR, p-4EBP1, GPX4, E-ca, and N-ca in brain sections.

Article Snippet: P28 peptide (sequence: LSTAADMQGVVTDGMASGLDKDYLKPDDC) was purchased from GenScript.

Techniques: Imaging, Control, Luciferase, Fluorescence, Isolation, Clinical Proteomics, Concentration Assay, Injection, Staining, Expressing

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

doi: 10.22038/IJBMS.2023.68331.14913

Figure Lengend Snippet: Shows how colon cancer cell lines’ cell cycles are affected by p28

Article Snippet: At the moment, bacterial peptides are gaining popularity as a cutting-edge method of treating cancer. p28 is well-known as a small peptide derived from Azurin which is a multitarget antitumor agent.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

doi: 10.22038/IJBMS.2023.68331.14913

Figure Lengend Snippet: Shows how p28 prevents colon cancer cells from migrating and encroaching

Article Snippet: At the moment, bacterial peptides are gaining popularity as a cutting-edge method of treating cancer. p28 is well-known as a small peptide derived from Azurin which is a multitarget antitumor agent.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

doi: 10.22038/IJBMS.2023.68331.14913

Figure Lengend Snippet: shows how p28, either alone or in combination with iRGD and 5-FU, inhibits the development of CT26 cells in xenograft nude mice

Article Snippet: At the moment, bacterial peptides are gaining popularity as a cutting-edge method of treating cancer. p28 is well-known as a small peptide derived from Azurin which is a multitarget antitumor agent.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

doi: 10.22038/IJBMS.2023.68331.14913

Figure Lengend Snippet: Shows the impact of p28 on indicators of oxidative, cytokines of inflammation, as well as genes that promote fibrosis

Article Snippet: At the moment, bacterial peptides are gaining popularity as a cutting-edge method of treating cancer. p28 is well-known as a small peptide derived from Azurin which is a multitarget antitumor agent.

Techniques:

Journal: Neuro-Oncology Advances

Article Title: The brain-penetrant cell-cycle inhibitor p28 sensitizes brain metastases to DNA-damaging agents

doi: 10.1093/noajnl/vdad042

Figure Lengend Snippet: p28 crosses the blood-brain barrier (BBB) in an in vitro 3D model. (A) Schematic of the in vitro 3D BBB model. Human brain vascular endothelial cells were cultured in the upper chamber of the Transwell system, and pericytes and astrocytes were cultured in the bottom chamber to form 2 distinct cell layers that mimic the transport properties of the BBB. (B) The apical (A) to basolateral (B) (influx) and B to A (efflux) transport of p28. (C) Permeability (influx) of p28 and TMZ in the BBB model. Mean ± SEM, ** P < .01.

Article Snippet: p28 peptide (LSTAA DMQGV VTDGM ASGLD KDYLK PDD, 2914 Da) and Scrambled-p28 (Scr, GDLSA DMPLD MGKVT VSGLD YAQAD TDK, 2914 Da) were synthesized by CS Bio, Inc at >97% purity.

Techniques: In Vitro, Cell Culture, Permeability

Journal: Neuro-Oncology Advances

Article Title: The brain-penetrant cell-cycle inhibitor p28 sensitizes brain metastases to DNA-damaging agents

doi: 10.1093/noajnl/vdad042

Figure Lengend Snippet: p28 crosses the BBB and preferentially localizes to Brain metastases (BMs). (A) Confocal images of the penetration of normal and cancer cells by p28. Human cancer cell lines (MDA-231BR, BCA-1, Mel-7, and A549) and normal cells (MCF-10A and fibroblasts) were cultured with Alexa Fluor 568-labeled p28 at 37°C for 2 hours, and images were obtained by confocal microscopy. Red, p28; blue, DAPI (nucleus). (B) MDA-231BR brain-specific metastatic triple-negative breast cancer, BCA-1 breast cancer, Mel-7 melanoma, or A549 lung cancer cells were injected into the left cardiac ventricle of athymic mice. ICG-labeled p28 was intravenously injected into the mice. Near-infrared fluorescence imaging of the ICG-p28 signal (gray) in coronal brain sections (yellow dotted line on the anterior-dorsal view) of mice injected with MDA-231BR, BCA-1 or Mel-7 cells or in the anterior-dorsal view of the brain of mice injected with A549 cells. H&E staining of brain sections confirmed the presence of BMs (Tu).

Article Snippet: p28 peptide (LSTAA DMQGV VTDGM ASGLD KDYLK PDD, 2914 Da) and Scrambled-p28 (Scr, GDLSA DMPLD MGKVT VSGLD YAQAD TDK, 2914 Da) were synthesized by CS Bio, Inc at >97% purity.

Techniques: Cell Culture, Labeling, Confocal Microscopy, Injection, Fluorescence, Imaging, Staining

Journal: Neuro-Oncology Advances

Article Title: The brain-penetrant cell-cycle inhibitor p28 sensitizes brain metastases to DNA-damaging agents

doi: 10.1093/noajnl/vdad042

Figure Lengend Snippet: p28 enhances the effects of radiation and TMZ. (A) Colonies were counted after MDA-231BR cells were treated with 50 μM p28 or 0.5 Gy radiation (IR) alone or in combination for 2 weeks. 50 μM Scrambled-p28 (Scr) was used as a control. Data are presented as the number of colonies per well, with representative images shown at the top. N = 3 per group. (B) Mel-7 cells were treated with 50 μM p28 or 100 μM TMZ alone or in combination and analyzed as described for (A). (C) Mel-7 cells were treated with 50 μM p28 or 0.5 Gy radiation (IR) alone or in combination and analyzed as described for (A). The percentage of apoptotic MDA-231BR (D, E) and Mel-7 (F, G, H, I) cells was quantitatively measured by flow cytometry. Data are presented as the mean±SEM. * P < .05, ** P < .01, *** P < .001, and **** P < .0001 (ANOVA).

Article Snippet: p28 peptide (LSTAA DMQGV VTDGM ASGLD KDYLK PDD, 2914 Da) and Scrambled-p28 (Scr, GDLSA DMPLD MGKVT VSGLD YAQAD TDK, 2914 Da) were synthesized by CS Bio, Inc at >97% purity.

Techniques: Control, Flow Cytometry

Journal: Neuro-Oncology Advances

Article Title: The brain-penetrant cell-cycle inhibitor p28 sensitizes brain metastases to DNA-damaging agents

doi: 10.1093/noajnl/vdad042

Figure Lengend Snippet: Induction of the p53-p21 axis. (A, B) MDA-231BR cells were exposed to PBS (NT), p28, radiation (IR), or the combination of p28 and radiation (p28/IR) for 24 hours (left panel). Mel-7 cells were exposed to p28, TMZ, or p28/TMZ for 24 hours (right panel). Proteins from whole-cell lysates (A) or from mitochondrial and cytosolic fractions (B) were separated by NuPAGE, and the indicated proteins were detected by western blotting. Actin and ATP5a were used as loading controls.

Article Snippet: p28 peptide (LSTAA DMQGV VTDGM ASGLD KDYLK PDD, 2914 Da) and Scrambled-p28 (Scr, GDLSA DMPLD MGKVT VSGLD YAQAD TDK, 2914 Da) were synthesized by CS Bio, Inc at >97% purity.

Techniques: Western Blot

Journal: Neuro-Oncology Advances

Article Title: The brain-penetrant cell-cycle inhibitor p28 sensitizes brain metastases to DNA-damaging agents

doi: 10.1093/noajnl/vdad042

Figure Lengend Snippet: p28 enhances the effect of radiation in a BM mouse model. MDA-231BR human triple-negative breast cancer cells stably expressing the luciferase gene (MDA-231BR-luc cells) were injected into the left cardiac ventricle of female athymic mice. After the development of brain metastases was confirmed, the animals were randomly divided into 3 groups. (A) Representative images of bioluminescence in the mice before and 1, 2, and 3 weeks after treatment with radiation (IR) alone or in combination with p28. (B) Quantitation of bioluminescence. Control: N = 5; IR alone: N = 10; p28/IR: N = 10. Mean±SEM. * P = .05 (IR alone vs. p28/IR). (C) Histological analysis of brain sections from representative mice in each group. Paraffin-embedded brain sections were stained with H&E and for the proliferation marker Ki67.

Article Snippet: p28 peptide (LSTAA DMQGV VTDGM ASGLD KDYLK PDD, 2914 Da) and Scrambled-p28 (Scr, GDLSA DMPLD MGKVT VSGLD YAQAD TDK, 2914 Da) were synthesized by CS Bio, Inc at >97% purity.

Techniques: Stable Transfection, Expressing, Luciferase, Injection, Quantitation Assay, Control, Staining, Marker

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

doi: 10.22038/IJBMS.2023.68331.14913

Figure Lengend Snippet: Shows how colon cancer cell lines’ cell cycles are affected by p28

Article Snippet: Peptide synthesis and purification ProteoGenix Inc. produced the p28 anticancer peptide (LSTAADMQGVVTDGMASGLDKDYLKPDD, 2914 Da) ( ) and the iRGD homing peptide (Ac-CRGDKGPDC-amide), a disulfide-based cyclic peptide, with >95% purity and mass balance.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

doi: 10.22038/IJBMS.2023.68331.14913

Figure Lengend Snippet: Shows how p28 prevents colon cancer cells from migrating and encroaching

Article Snippet: Peptide synthesis and purification ProteoGenix Inc. produced the p28 anticancer peptide (LSTAADMQGVVTDGMASGLDKDYLKPDD, 2914 Da) ( ) and the iRGD homing peptide (Ac-CRGDKGPDC-amide), a disulfide-based cyclic peptide, with >95% purity and mass balance.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

doi: 10.22038/IJBMS.2023.68331.14913

Figure Lengend Snippet: shows how p28, either alone or in combination with iRGD and 5-FU, inhibits the development of CT26 cells in xenograft nude mice

Article Snippet: Peptide synthesis and purification ProteoGenix Inc. produced the p28 anticancer peptide (LSTAADMQGVVTDGMASGLDKDYLKPDD, 2914 Da) ( ) and the iRGD homing peptide (Ac-CRGDKGPDC-amide), a disulfide-based cyclic peptide, with >95% purity and mass balance.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

doi: 10.22038/IJBMS.2023.68331.14913

Figure Lengend Snippet: Shows the impact of p28 on indicators of oxidative, cytokines of inflammation, as well as genes that promote fibrosis

Article Snippet: Peptide synthesis and purification ProteoGenix Inc. produced the p28 anticancer peptide (LSTAADMQGVVTDGMASGLDKDYLKPDD, 2914 Da) ( ) and the iRGD homing peptide (Ac-CRGDKGPDC-amide), a disulfide-based cyclic peptide, with >95% purity and mass balance.

Techniques: